here's why I put a little fun bounty on it: the inverse study
a completely 100% unassisted cycle that self created in 4 mos. demo'd on api, quite clearly able to process ammonia.
it seems one that was blast fed and had hyperconcentrated bacteria sure can't go back to sterile...surfaces underwater get fed via natural contamination means in the home they're running in
perhaps a positive pressure microbiology lab could keep one sterile, not a home though/
it's not been formally studied. it's worth a little incentive to get real digital data on the matter, it's uncharted territory.
MSteven's posts
I have never seen that documented in twenty years of daily online reefing. Unassisted cycling happens sooner than your time frame, tbd, but I'd have bet the farm it'd take ninety days. I'm so happy to see that Cycling charts were written for freshwater You're providing data for a saltwater...
www.reef2reef.com
despite the trolling, I'm convinced. It lines up with all known rules of cycle biology, after all in the 80s 100% of our freshwater setups were unassisted cycles using no live materials. tap water, dechlor, 30 days, cycled, no dosers no ammonia no feed. literally the home did it
reefs are the same, it takes longer and the original strains that can move ammonia in the first 4 months are replaced by actual marine strains of permanent nitrifier bacteria as soon as reef additions start/ fish or coral imports.
a good experiment will have 2 or 3 prepped buckets of rocks.
they're stagger-tested. once you hit one with ammonia to test oxidation rebound time, that set is ruined for the test bc they were fed directly.
the other two buckets can be +2 mos staggered out for starvation testing.
Heck I'd accept API data on the matter but would pay less lol. constraints: no 24 hour rebound required due to the ten day lag time we show on page 1, post #2
and no dosing to 2 ppm either for the load test, that messes up everyone's api. MSteven tested it exactly right, barely enough ammonia to move the color one gradient darker is correct. I'd pay 1/3 bounty for solid api data showing hard stalls days after the test load.
seneye would make it so much easier though, in 15 mins the entire process could be measured accurately and charted and considering it's benched off a real reef, nobody can deny what's measured even me if it shows a starve.