@howaboutme
I must introduce the greatest conundrum in reefing since you are about 90% close to testing it.
The timeframe for a truly unassisted marine cycle is not known.
no reefing guru has listed how long it takes, the one part of your experiment that changes it from unassisted into assisted is the rehydrated organics, those are directly boosted bacterial feed in every possible way. total feed, yummy feed when rehydrated and broken down by non filtration bacteria to lend ammonia into the machinery for nitrification establishment.
But a true unassisted cycle is dry sand, totally dry inert rock, sat in saltwater open topped circulated and heated, and then wait
X number of days to carry a reasonable starting bioload, with decent current lets say it can oxidize .5 ppm ammonia nh3 very quickly max. that's enough for legit cycling call, nobody knows how long this takes.
The cycling chart we use is for freshwater ~ as dry expoxy gravel, tap water with start right, fake palm trees was every tank I owned as a kid and if you waited 30 days as told, your gouramis and swords lived.
if you cheated, and bought them at one weeks wait, they all died and you went back to the pet store and they caught you, in that order lol.
Nobody knows how long truly unassisted marine cycling to .5 ppm takes to set in for a given set of dry goods.
New science, out there for the tanking. an article is needed on this, stat.
*the seed sources for freshwater work are everywhere, in the garden we know, in the air as dust etc/vectors everywhere
marine is different, especially if in Kansas
other bacteria get in and simply handle the salt, we think, establishing an initial food web that really does handle some ammonia on X date and then over time and alternation of generations new bacteria lay down into place for long term establishment, even in a reef in Kansas. unassisted. Ill be happy one day to have this answer, it eludes us for now.
B