Yes - there is three rollers. If you have your worst bad hair day ever and only a doser pump connected - they can theoretical stop exactly in that position (we are talking in µm here) that no roller squeeze the internal tube at all/very little and there is a tiny, tiny, tiny chance that you get a backflow. If the dosing pump is connected to a KH or Ion director - I´ll think the chance is lesser than that MAC and Windows will be compatible one day
Never the less - I always have my pumps over the actual water level

of other concerns. The joint between the suction tube (from the aquarium) and the pump head can can come loose. I have one pump where i can theoretical empty my 300 L aquarium into a 500 mL bottle. As chicken is my middle name - I have a check valve on the pressure side. Mostly because there is a chance that the tube can loosen in the joint that comes after the pump head and a backflow will be created. To use a check valve for the sample suction tube of both ION and KH director can affect the result - I will not recommend that. I´ll think there is a real risk of disaster - but not because a back flow through the pump head but because that the sample tube can be loose from the intake of the pump head. If you can fix a hose clam that fix both the 6 mm suction tube and the 4 mm pump head tube - you will be safe for that
Sincerely Lasse