Tank of Death :(

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DeeBlue

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Dear R2R Community,

im hoping for your help, since we are out of options, and just dont know how to continue on atm.

Our 320g mixed reef was thorn apart this april, because some fish presented with symptoms of velvet. Also some deaths occured in this acute outbrake. I must say at this point that velvet could not be confirmed using body/gill swipes, but at least cryptocarion was present for sure.

It is worth mentioning we had several fish since october we lost a few days after introduction into the tank, without obvious symptoms. We didnt QT fish at that time.

As answer to the suspected velvet outbreak all rockwork/corals were taken out, the fish caught and treated with cupramine (fish group #1) or CP (fish group #2) in hospital tanks/QT tanks.

Fish improved, and were back to obvious full health.

The DT was left fallow for over 80 days. In this period a lots of coral (the tank was rich in SPS/LPS) deteriorated. Professional water analysis was conducted (ICP-OES), water parameters turned out to be great (despite some elevated aluminium and decreased strontium). We figured, that the corals might actually be dying because a)the absence of fish and b) the stress of removing them from the tank, during the "catch all fish" session.
.
Anyways, fish matter more to us than coral, so we continued with the fallow period (full 80 days). We lost about 60% of coral during this time, and the deterioration of coral seems to move on.

On saturday some fish of fish group #1 (1 Naso tang, 1 Scarus wrasse, 1 N. Decora, 1 Banggai cardinal) were returned to DT after careful acclimatization. Fish were feeding extremely well back in the DT, and looked very happy to be back in DT again.

The happiness lasted for 24 hours. After that the scarus wrasse concerned us, because she was sleeping outside a hiding spot. It was dead the next morning. Within the following day our large Naso (a really personal fish, i could cry right now...) died. Yesterday we found the N. decora on the sand bed. She looked almost like paralyzed and was easy to catch. No abnormalities on the skin. No heavy breathing. We got her out and put her in heavily aerated new water, but she died within a few hours. Microscopic examination didnt show parasites on the skin.

The last survivor in the DT is the Banggai cardinal. He doesnt eat, looks skin-wise 100% normal, but breaths in a strange way. The rate isnt increased, but the breathing seems to be "deeper".

I have absolutely no idea what is going on. Maybe the coral deaths and the fish deaths are interconnected.

What was ruled out so far:

- Ammonia
- Lack of oxigen
- Toxic inorganics (by ICP-OES testing)
- Velvet/Crypto (seems it seems impossible after the QT/fallow tank period to have fish because of this disease within ~ 30 hours)
- Poisoning by household cleaners, etc (we are extremely careful in this respect)

Yesterday is also tested voltage in the tank, but this seems to be a topic with very opposing opinions. I tend to stick with the "bird on a wire" argument. We could detect 70V AC againt ground, with about 1.3 mA current flow. The voltage seems to arise from all pumps together, disconnecting each single pump drops the voltage by a few volts, but there doesnt seem to be a single cause. I would be suprised if this would be the reason for our "Death tank". Experience, anyone?

Im running some DC devices (1 gyre, 1 tunze and 3 aqua medic powerheads as well as the skimmer pump and pump for the UV-C), and furthermore some AC devices like the return pump, the heater and and 2 pumps that supply the media reactor and the frag tank.

None of the equipment seems damaged.

Im so puzzled. We would like to return the other fish from QT, but there is no way putting them into the Death Tank without finding the reason.

#ReefSquad, please help!!!

All the Best!
Dee
 
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How deep is your sandbed? And how does your sand feel? Is it like sand at the beach or is it silty?

What test did you use to determine ammonia?

How did you determine oxygen is fine?

The symptoms and timing correlate to oxygen or how the fish uptake oxygen. This would lead me to think that the sandbed is around 3+" and there was a anaerobic zone that was disrupted, either by a fish disturbing it or a rock being moved.
 
How deep is your sandbed? And how does your sand feel? Is it like sand at the beach or is it silty?

What test did you use to determine ammonia?

How did you determine oxygen is fine?

The symptoms and timing correlate to oxygen or how the fish uptake oxygen. This would lead me to think that the sandbed is around 3+" and there was a anaerobic zone that was disrupted, either by a fish disturbing it or a rock being moved.

Hi!
Thanks for your answer!

My sandbed is greatly reduced, we suctioned off most of the sand during the last weeks (last disturbance of sand more than a week ago), because it was recommended to us to stop the coral problems. Sandbed was always less then 1 inch in thickness. The sand was never silty, it was always free flowing, and when removing the sand i didnt have any feeling there was a problem with the sand. Now there is only some sand left (under the rocks, where it was not possible to remove it). I also never noticed a "foul smell" that i would expect from rotting sand.

Ammonia is monitored using a SeaChem badge.

Regarding Oxygen im having very high flow at the surface, and in addition a skimmer with high air intake. - So oxygen shortness would very much surprise me. I dindt have the possibility to actually measure O2.

Cheers,
Dee
 
Is there a media reactor or refugium?

There is a media reactor containing some Fe-oxide based phosphate remover. Yeysterday ive also added some carbon to it. Its the "column" type media reactor. There is also a frag tank (around 55 gallons) that is fed from the same sump. This tank contains also live rock and sand. The sand is this tank was not removed.

Following your theory that something is consuming up oxygen, or something blocking oxygen uptake: What steps should i take to rule that out? Is there a reasonable sensitive O2 test?

Thanks for your help!
 
There is a media reactor containing some Fe-oxide based phosphate remover. Yeysterday ive also added some carbon to it. Its the "column" type media reactor. There is also a frag tank (around 55 gallons) that is fed from the same sump. This tank contains also live rock and sand. The sand is this tank was not removed.

Following your theory that something is consuming up oxygen, or something blocking oxygen uptake: What steps should i take to rule that out? Is there a reasonable sensitive O2 test?

Thanks for your help!
With that much surface area, skimming, and agitation, I don't think there is a shortage of o2, but rather either ammonia or hydrogen sulfide. I don't trust ammo badges and would be curious to see an ammonia reading from a non api test kit.

If there is no medication in the system you could try prime or another ammonia neutralizer.
 
With that much surface area, skimming, and agitation, I don't think there is a shortage of o2, but rather either ammonia or hydrogen sulfide. I don't trust ammo badges and would be curious to see an ammonia reading from a non api test kit.

If there is no medication in the system you could try prime or another ammonia neutralizer.

I have also ammonia tests from JBL and Salifert in place, all readings negative. Concerning the hydrogen sulfide, i cant detect anything by smell, so i would rule that out.

Anyone ideas regarding the electricity? Is 1.3 mA@70V towards ground problematic?

Best,
Dee
 
I have also ammonia tests from JBL and Salifert in place, all readings negative. Concerning the hydrogen sulfide, i cant detect anything by smell, so i would rule that out.

Anyone ideas regarding the electricity? Is 1.3 mA@70V towards ground problematic?

Best,
Dee
Issues with electricity have always been slow acting, unless it's enough to give you a potent shock. I've found it exhibits as hlle over months rather than overnight death.
 
Have you taken gill scrapes of the newly deceased to check for parasites there? Including flatworms/Monogeneans? You can also check for flukes by performing a FW dip (even on a dead fish): https://www.reef2reef.com/threads/freshwater-dip.248898/

Hello!

Unfortunately, i didnt perform a FW dip on the dead fish. Also Gills were not examined, But fish have been treated twice with praziquantel (in addition to copper) while in QT, and the DT was left fallow for 80 days. Also which parasite could take down a very healthy feeding fish within 48hours?

Is it possible bacteria do that? I could imagine that fishs immune system is rather down after copper and spending a lot of time in a more or less sterile QT?

Otherwise i can just think of a toxic substance...

Best,
Dee
 
Do you have a chloramine block in the RODI?

What about external contaminants? Any air sprays or any chance something could have gotten into the tank?
 
Do you have a chloramine block in the RODI?

What about external contaminants? Any air sprays or any chance something could have gotten into the tank?

No chloramine block, in fact ive never heard of such a thing. Water here, straight from the tap, is very high quality and not chlorinated.

No air sprays, deodorants and perfumes are used just in the bathroom, about 8 away from the tank (around a corner).

Thanks for your suggestions!

Best,
Dee
 
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Also which parasite could take down a very healthy feeding fish within 48hours?

Velvet; although I will point out that a few Monogeneans are prazi resistant. These can only be eradicated via formalin or hypo.

Is it possible bacteria do that? I could imagine that fishs immune system is rather down after copper and spending a lot of time in a more or less sterile QT?

Yes, a gram negative infection can kill extremely fast. But even if the infection was occurring internally, some odd discoloration (red, brown) should still show through the skin. Of course, a necropsy would be more revealing.

Otherwise i can just think of a toxic substance...

How are the remaining corals looking? A toxin should be affecting them too. I do find it odd that you lost so many corals during a fallow period. Did your nitrates just bottom out and you never could raise it?
 
More info on Marine Velvet Disease: https://www.reef2reef.com/threads/velvet-amyloodinium-ocellatum.217570/

In particular, are any of your fish displaying any of these key behavioral symptoms of velvet:
  • Reduced or complete loss of appetite.
  • Heavy breathing, rubbing, flashing, head twitching, erratic swimming behavior (unfortunately velvet shares all these same symptoms with ich & gill flukes.)
  • Swimming into the flow of a powerhead (unique to velvet).
  • Acting reclusive (velvet causes fish to be sensitive to light).
 
Hi Humblefish,

suspected velvet was the reason why the fish went into HT/QT more then 2.5 months ago. Treatment using copper was working very well, the deceased fish now also didnt show any symptoms of velvet.

Gram positive bacteria could be the cause, but no discoloration was visible. Are those bacteria usually in the water column? Did you encounter problems sometimes, bringing fish back into DT from a sterile QT?

How are the remaining corals looking? A toxin should be affecting them too. I do find it odd that you lost so many corals during a fallow period. Did your nitrates just bottom out and you never could raise it?

Im still having issues with corals. LPS look very weird, and SPS (Stylophora for example) are still degrading. Others (interestingly blue acroporas & stylophora pistillata dont show any symptoms). I think there is some connection between the coral and fish losses.

A lot of water was changed during the last weeks. This is a very recent analysis: http://lab.atiaquaristik.com/share/68a8bafb165c4e19eb13 (sorry, german mainly)

Best,
Dee
 
Gram positive bacteria could be the cause, but no discoloration was visible. Are those bacteria usually in the water column? Did you encounter problems sometimes, bringing fish back into DT from a sterile QT?

Gram positive bacteria are almost always present in the water, but a healthy fish's natural immune system usually keeps such at bay. Plus, there would have to be a wound or small hole for the bacteria to infect. So visible symptoms of a bacterial infection will almost always manifest. Unless a mild one is occurring internally.

Im still having issues with corals. LPS look very weird, and SPS (Stylophora for example) are still degrading. Others (interestingly blue acroporas & stylophora pistillata dont show any symptoms). I think there is some connection between the coral and fish losses.

IMO; this strongly suggests an environmental problem which is negatively impacting both your fish and corals. Whether it is a toxin or stray voltage or something else, I cannot say.....
 
IMO; this strongly suggests an environmental problem which is negatively impacting both your fish and corals. Whether it is a toxin or stray voltage or something else, I cannot say.....

This is what i am thinking aswell. The only possibility is organic toxins, since ICP-OES ruled out inorganics.

This evening the banggai cardinal showed up again, seems he was hiding in the reef. We were thinking he is dead aswell. Tough fish - last survivor! His behaviour is still strange, including very deep breathing. Here is a short video we just made:


Hopefully he makes it. Any suggestions?

Have a good weekend!
Dee
 

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