Anecdotes in favor of a fish-in cycle with a bottle of bacteria...
At the risk of getting absolutely skewered...I have cycled two tanks with two clowns and a tail spot blenny (all at once) and a bottle of Dr. Tim's. The ammonia level barely shifted from yellow to slightly greenish yellow on a Red Sea test kit...and it hit zero in less than 48 hrs. I couldn't tell you what the level actually was because the color barely changed. It could have been within the range of error on the test! I kept a close eye on it and I was ready to do a massive water change if anything got out of hand. That would have fixed the problem quickly, and I believe being ready to ameliorate issues if things go awry is all that is necessary to be responsible when cycling a tank with fish...even if the tank is technically cycled when you add the bacteria (more on this in a second).
Before I just threw a fish in the tank, this is the logic I used to come to my conclusion:
1. The INSTRUCTIONS ON THE BOTTLE of Dr. Tim's say that a fish can be added right away, since adding the bacteria to the tank IS actually cycling the tank, because cycling the tank is really about creating your bacterial population. This just does it more quickly.
And I can only assume the tiny ammonia spike is from the nitrifying bacteria adjusting to keep up with the bioload. Why would Tim tell us to throw a fish into a tank with his product knowing it could kill the fish? That wouldn't be great for business now would it...
2. When I set up my first QT tank as a complete newbie, I was flummoxed about how to quickly "cycle" the tank if I didn't have any biomedia from another tank. And then I asked myself the question: why would a little sponge full of biomedia in a HOB filter "cycle" the tank any better than a bottle of bacteria soaking the same sponge? I also knew that if I spent time cycling the QT tank with bottled ammonium chloride, I would end up with high nitrates AND there wouldn't really be much surface area for the bacteria to colonize for the size of the tank, based on the recommendations for ~1lb of live rock per gallon of water. I didn't want to have to do a huge water change before I added the fish, and I knew high nitrates plus copper and other meds would stress the fish more than the meds alone. Again...I had no problems and there was pretty much zero ammonia spike with three fish in a 10g tank. All three of them sailed through QT.
3. I believe I double checked this with
@brandon429 to make sure I wasn't being barbaric. This was about two years ago.
Side note:
I would argue that using ammonium chloride doesn't set you up for long term success because it takes longer to cycle the tank and the higher ammonia concentrations from dosing vs. what's produced by a few small fish cause sky-high nitrate levels. Not great for a sterile tank started with dry rock, no? Seems like a recipe for an algae outbreak to me...
More evidence: cycling a tank with maricultured premium live rock...
In a completely different scenario, with a brand new tank, I cycled the tank with KP Aquatics premium rock.
I won't pretend this situation wasn't stressful, and it required a lot of large water changes over a week, because in this case, the initial die off caused the ammonia to rise to much higher concentrations than the bottle of bacteria and the fish (not off the charts, but still significant).
The reason this was stressful was because I had so much LIFE on the rocks. Crabs, brittle stars, fan worms, snapping shrimp, copepods and amphipods...the list goes on. Obviously I wanted to preserve as much life as possible (this is the point of buying live rock! And I'm not a monster), so I monitored very closely and did 30% water changes every two days. I only lost one starfish out of MANY and a single small snapping shrimp out of two. Obviously I can't tell you whether the ammonia or shipping stress killed them, but I think these animals are hardier than we think. The journey alone is harrowing.
The cycle was over in a week, and I have so many different crabs it's actually a problem lol.
That's my opinion (ahem...diatribe) on the subject, and while it's anecdotal, I think we should listen to Dr. Tim, even if he has unfortunately confused the situation by providing fishless cycle instructions and materials.