http://reef2reef.com/threads/the-of...ead-aka-one-against-many.230281/#post-2681445
That will cover all bacteria questions
Cleaning time
I'm about to take apart my whole reef (ten yrs old) in the exact steps above and rinse my sb with tap water

right now, 100% new clean run...change water is heating up to 78 as we speak.
Just quick pass tap, final rinse is sw and the sand remains viable filter, this does not sterilize. Everybody has tap water that pumps out live bacteria, in spite of chlor...and that's from the plant fed report...pipe scum and o ring pass by adds lots more bacteria to your cup of lemonade, search this out to see.
The CFU report from your local water supply annual reports shows the bacteria colony approximations coming to your tap
Translated into sandbed work, it means you have a dwell time such that five mins rinse harms nothing.
After parted out rinse I'll then put back together and skip cycle. The reef is tiny so this is easy, I do it 2-3 times a year so I can feed heavy
Altering the bac takes meds or desiccation not any rinsing we do or short air exposure.
The biology is the same regardless of tank size...just more work for large tankers and they have fish to isolate
Pics coming
I don't even put my corals in water they sit on the counter on a dinner plate. Live rock too, takes about 15 mins then they go back in.
**some people have remote refugiums or special setups where you don't rinse. If your sandbed is one of those, don't rinse lol